Evaluation of the completeness of the Hydra-bo transcriptome. A) Construction of the Hydra-meta transcriptome, which contains an unredundant assembly of the Hydra-bo and predicted sequences (pred-CA and pred-RP). B) Saturation analysis of transcripts representation (y-axis) with increasing numbers of sampled reads (x-axis). The Hydra-meta transcriptome was used as a reference. Subsets of randomly sampled Illumina reads were collected and mapped to the reference using Bowtie. The numbers of mapped reads (hits) were counted for each reference sequence.
Wenger and Galliot BMC Genomics 2013 14:204 doi:10.1186/1471-2164-14-204