Assembly procedures of the de-novo (dn) and genome-assisted (ga) Hydra transcriptomes. A, B) Schemes showing the de-novo (dn) assembly process of Hydra RNAseq reads (A), and the genome-assisted (ga) and “best of” (bo) assembly processes of Hydra transcriptomes (B). Numbers of sequences are indicated at each step. For steps 2a and 2b, artificial Phred quality values were produced for the dataset called “Contigs” and the sequences were duplicated in order to further correct frameshifts due to 454 sequencing (see Methods section for details).
Wenger and Galliot BMC Genomics 2013 14:204 doi:10.1186/1471-2164-14-204