Human CSNK2B, LY6G5B and CSNK2B-LY6G5B chimeric protein characterisation. Schematic representation of A) V5 and Hisx6 double tag strategy. V5 (red box) and Hisx6 (yellow box) epitopes are shown. Exon numbers are indicated. B) Western-blot analysis showing in vitro expression of CSNK2B, LY6G5B and CSNK2B-LY6G5B chimera double tagged proteins. GAPDH expression is shown as internal control. C) Immunofluorescence experiments in permeable (P) and non permeable (NP) conditions showing CSNK2B, LY6G5B and CSNK2B-LY6G5B chimeric protein cellular distribution.
Hernández-Torres et al. BMC Genomics 2013 14:199 doi:10.1186/1471-2164-14-199