Table 4

Summary of knock-out results for candidate regulators in the Ribi group
ΔSDS24 ΔPBF2 ΔREI1 ΔRAI1 ΔKAP114 ΔSDS24 HS ΔPBF2 HS ΔREI1 HS ΔRAI1 HS ΔKAP114 HS
% targets affected 4.7% 4.7% 74.4% 83.7% 4.7% 7.0% 79.1% 79.1% 86.0% 60.5%
max change 1.691 1.983 2.686 2.934 1.838 1.531 5.117 5.700 5.977 3.847
avg. change 1.168 1.245 1.641 1.824 0.962 1.174 2.206 2.241 2.417 1.641
# controls affected 0 0 3 2 1 0 1 5 3 1
p-value 1 1 8.6e-6 2.3e-8 1 0.55 1.3e-8 6.1e-5 7.6e-8 2.6e-5

Genes with expression levels greater than 1.5 times the wild type, or less than 2/3 the wild type, levels are considered to be affected by the knockout. The max change and average change rows represent the fold change in the expression level compared to the wild time. HS denotes that the yeast were heat shocked before extracting RNA. There were 20 total control genes. The p-value represents a Fisher’s exact score between the genes affected in the target and control groups.

Curtis et al.

Curtis et al. BMC Genomics 2013 14:196   doi:10.1186/1471-2164-14-196

Open Data