Validation of selected DEGs by qRT-PCR. Three up- and downregulated genes detected by RNA-seq (blue) were selected from skin (A) and brain (B), respectively, and qRT-PCR (red) was performed on the same RNA-samples. Except for VDRB, fold changes (log-transformed) did show good agreement between RNA-seq and qRT-PCR.
Petzold et al. BMC Genomics 2013 14:185 doi:10.1186/1471-2164-14-185