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Open Access Research article

Integrated analysis of cytochrome P450 gene superfamily in the red flour beetle, Tribolium castaneum

Fang Zhu, Timothy W Moural, Kapil Shah and Subba Reddy Palli*

Author Affiliations

Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY, 40546, USA

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BMC Genomics 2013, 14:174  doi:10.1186/1471-2164-14-174

Published: 14 March 2013

Additional files

Additional file 1:

List of P450s in Tribolium castaneum.

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Open Data

Additional file 2:

Identity matrix of genes in the CYP6BQ cluster illustrating percentage identities among 12 cluster genes.

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Open Data

Additional file 3:

Intron-exon constructions of CYP6BQ cluster genes. Shaded bars and lines represent gene exons and introns to scale, respectively.

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Open Data

Additional file 4:

Differential expressions of clustered genes between resistant QTC279 and susceptible LBS strains. The expression levels were normalized by rp49, the endogenous control. All data was averaged by three replicates. The result was shown as the mean + SE.

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Open Data

Additional file 5:

Induction of clustered genes in QTC279 strain following treatment of deltamethrin. The expression of these genes was analyzed by qRT-PCR as described in the methods. Relative expression level was normalized by rp49. The result was shown as the mean ±SEM (n= 3). There was no significant difference in the level of expression among samples with the same alphabetic letter (i.e. a, b, c) (P < 0.05).

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Open Data

Additional file 6:

Sequence alignment for CYP6BQ8, 9, 10, 11. Within 4.5 Å of deltamethrin, the predicted CYP6BQ8, CYP6BQ9, CYP6BQ10 and CYP6BQ11 catalytic sites contact with residues which were labeled in red color.

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Open Data

Additional file 7:

CYP6BQ cluster genes binding mode energy and distance.

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Open Data