Open Access Research article

De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel

Vera Hershkovitz1, Noa Sela2, Leena Taha-Salaime134, Jia Liu5, Ginat Rafael1, Clarita Kessler1, Radi Aly3, Maggie Levy4, Michael Wisniewski5 and Samir Droby1*

Author Affiliations

1 Department of Postharvest and Food Sciences, ARO, the Volcani Center, Bet Dagan, 50250, Israel

2 Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, 50250, Israel

3 Department of Plant Pathology and Weed Research, the Volcani Center, Newe-Yaar Research Center, Ramat Yeshai, 30095, Israel

4 Department of Plant Pathology and Microbiology, the Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, Revovot, 76100, Israel

5 U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, Kearneysville, WV, 25430, USA

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BMC Genomics 2013, 14:168  doi:10.1186/1471-2164-14-168

Published: 12 March 2013

Additional files

Additional file 1:

A fasta file containing all Unigene sequences of Metchnikowia fructicola transcriptome.

Format: TXT Size: 5.2MB Download file

Open Data

Additional file 2:

Annotation of all Metschnikowia fructicola unigenes: the gene description, go terms associated and proscan analysis of every transcript.

Format: XLSX Size: 887KB Download file

Open Data

Additional file 3:

Mapping of Metschnikowia fructicola de-novo assembled transcript to Metschnikowia fructicola genome draft genbank accession number ANFW01000000.

Format: XLSX Size: 785KB Download file

Open Data

Additional file 4:

The differentially expressed genes in Metschnikowia fructicola interaction with Penicilium digitatum (Mf-Pdig) and interaction with fruit (Mf-fruit) and with fruit (p < 0.05).

Format: DOCX Size: 62KB Download file

Open Data

Additional file 5:

Summary of differential expressed genes in Metschnikowia fructicola interaction with Penicillium digitatum and interaction with fruit (p < 0.05) involved in the response to stresses (chemical GO:042221), oxidative (GO:006979), osmotic (GO:006970), heat (GO:009408), starvation (GO:042594), DNA damage stimulus (GO:006974).

Format: DOCX Size: 24KB Download file

Open Data

Additional file 6:

Summary of differential expressed genes in Metschnikowia fructicola interaction with Penicillium digitatum and interaction with fruit (p < 0.05) involved in transport (transmembrane GO:055085), ion (GO:006811), carbohydrate (GO:008643), lipid (GO:006869), amino acid transport (GO:006865).

Format: DOCX Size: 28KB Download file

Open Data

Additional file 7:

Summary of differential expressed genes in Metschnikowia fructicola interaction with Penicillium digitatum and interaction with fruit (p < 0.05) involved in metabolic (lipid GO:006629), (vitamin GO:006766), (cofactor GO:051186), (cellular amino acid GO:006520) processes.

Format: DOCX Size: 24KB Download file

Open Data

Additional file 8:

The primer sequences used in qPCR.

Format: DOCX Size: 15KB Download file

Open Data