Open Access Research article

Characterization and differential expression of microRNAs in the ovaries of pregnant and non-pregnant goats (Capra hircus)

Xiao-Dong Zhang12, Yun-Hai Zhang12, Ying-Hui Ling12, Ya Liu12, Hong-Guo Cao12, Zong-Jun Yin12, Jian-Ping Ding12* and Xiao-Rong Zhang12*

Author Affiliations

1 Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, No. 130 Changjiang west road, Hefei 230036, P. R. China

2 College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang west road, Hefei 230036, P. R. China

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BMC Genomics 2013, 14:157  doi:10.1186/1471-2164-14-157

Published: 7 March 2013

Additional files

Additional file 1:

Distribution of counts of the sequencing results originating from known RNA classes in non-pregnant and pregnant libraries.

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Additional file 2:

Conserved miRNAs and miRNA*s in the ovaries of non-pregnant goats. The miRNA: miRNA* duplexes were marked in red font.

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Additional file 3:

Conserved miRNAs and miRNA*s in the ovaries of pregnant goats. The miRNA: miRNA* duplexes were marked in red font.

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Additional file 4: Figure S1:

The length distribution of unannotated sequences in non-pregnant and pregnant libraries. Figure S2. The stem loop structures of precursors of predicted miRNA candidates. The red line parts indicate the mature sequences. (A) Non-pregnant; (B) Pregnant. Figure S3. Analysis workflow of the Solexa sequencing results.

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Additional file 5:

Identification of goat putative miRNAs from goat EST sequences.

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Additional file 6:

Differential expression of conserved miRNAs and miRNA*s in the ovaries of non-pregnant and pregnant goats. (1). -std. represents normalized expression level of miRNA in a sample. Normalized expression = Actual miRNA count/Total count of clean reads*1,000,000. (2). Sig-label: ** represents fold change (log2) > 1 or fold change (log2) < -1, and P-value < 0.01; * represents fold change (log2) > 1 or fold change (log2) < -1, and 0.01 ≤ P < 0.05; None represents others. Fold change = log2 (Pregnant std. / Non-pregnant std.) (3). miRNAs in red font used for the q-PCR analysis.

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Additional file 7:

Primer sequences for q-PCR experiments.

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