Additional file 4: Figure S3.
Absolute quantification of miR-30a copy number. A. Standards of synthetic miR30a oligonucleotide were used at known nanomolar concentration in a TaqMan qRT-PCR assay. Amplification plots are shown in the left panel, and standard curve is shown in the right panel, with corresponding efficiency and correlation values. Biological samples were run simultaneously with known miR30a standards in TaqMan qRT-PCRs. After accounting for the different dilutions and original number of cells, absolute number of copies per cell was obtained for parental MCF7 cells compared to MCF7-derived mammospheres (B), and MCF7 cells treated with different experimental conditions (MCF7 controls, miR30a overexpression and knock-down) (C). P values under 0.05 (two-tailed student t test) are represented with an asterisk (*).
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Ouzounova et al. BMC Genomics 2013 14:139 doi:10.1186/1471-2164-14-139