Additional file 3: Figure S2.

Validation of miRNA expression using Illumina platform. miRNA profiling was validated using Illumina bead arrays in independent biological replicates of MMO and parental MCF7 cells. A. Scatter plot between 2 technical replicates indicating a proper correlation. B. Unsupervised cluster analysis and heat-map including all probes. C. Volcano plots of fold change (represented in Log2 ratio in the x axis) vs. P value (represented in Log10 ratio in the y axis). Volcano plots indicate a subset of miRNAs downregulated in MMO, respective to MCF7 cells. D. Expression of selected miRNAs significantly downregulated in MMO, relative to parental MCF7 cells.

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Ouzounova et al. BMC Genomics 2013 14:139   doi:10.1186/1471-2164-14-139