Table 4

Summary of BLAST searches of Amblyomma americanum against Ixodes scapularis
UniProtKB A. americanum ESTs
A. Datasets for I. scapularis Annotation No Annotation Totals
ESTs Match 2,997 457 3,454 (24.1%)
No Match 1,121 9,735 10,856
Predicted Peptides Match 3,413 179 3,592 (25.1%)
No Match 705 10,013 10,718
Contigs Match 1,724 642 2,365 (16.5%)
No Match 2,394 9,550 11,944
Singletons Match 3,279 845 4124 (28.8%)
No Match 839 9,347 10,186
I. scapularis ESTs
B. Datasets for I. scapularis Totals
Predicted Peptides Match 128,738 (66.2%)
No Match 70,722
Contigs Match 72,465 (37.3%)
No Match 121,995
Singletons Match 105,183 (54.1%)
No Match 89,277

(A) Amblyomma americanum EST sequences matching Ixodes scapularis genomic datasets and (B) I. scapularis EST sequences matching other I. scapularis datasets. Ixodes scapularis datasets include ESTs, predicted peptides, singletons, and assembled contigs. (A) Amblyomma americanum results are categorized according to UniProtKB annotation status (annotation, no annotation) and I. scapularis match status (match, no match). The proportion of the A. americanum ESTs with a match is provided in parentheses for each I. scapularis dataset. (B) BLAST searches of I. scapularis ESTs (N = 194,460) against the three other I. scapularis datasets were then conducted to evaluate the quality of these datasets. These are classified according to match status. The proportion of I. scapularis ESTs with a match is provided in parentheses.

Gibson et al.

Gibson et al. BMC Genomics 2013 14:135   doi:10.1186/1471-2164-14-135

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