Distribution of the nucleotide lengths of open reading frames for annotated and unannotated EST sequences. Annotated ESTs are shown in black and unannotated in white. Open-reading frame lengths were predicted using the OrfPredictor component of the Transcriptome Analysis Pipeline of the Integrative Services for Genomic Analysis (ISGA) at Indiana University’s Center for Genomics and Bioinformatics. Annotation was determined by a BLAST search of the A. americanum EST library against the UniProtKB protein database.
Gibson et al. BMC Genomics 2013 14:135 doi:10.1186/1471-2164-14-135