Table 5

Microorganisms and plasmids used in this study
Strains Relevant properties Source
Escherichia coli
JM109 Cloning host, genotype:endA1, recA1, gyrA96, thi, hsdR17 (rk, mk+), relA1, supE44, (lac-proAB), [F’ traD36, proAB, laqIqZΔM15] Promega
JM109 (DE3) Expression host, genotype:endA1, recA1, gyrA96, thi, hsdR17 (rk, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F’, traD36, proAB, lacIqZΔM15], lDE3 Promega
Bacillus subtilis
WB800N Secretion host with resistance to neomycin, genotype: nprE aprE epr bpr mpr :: ble nprB :: bsr vpr wprA :: hyg cm :: neo; NeoR Mo Bi Tec
Streptomyces virginiae
IBL14 Wild type Our lab
Plasmids
pET22b Expression vector in E. coli Novagen
pHCMC05 Shuttle plasmid BGSC
pET22b-svh01-svf09 The fragment of svh01 and svf09 were digested with NdeI/EcoRIand EcoRI/Hind Ш, respectively, and ligated into the NdeIand Hind Ш sites of pET22b This study
pHCMC05-svu022 The gene of svu022 digested with BamHI/SmaIligated into BamHI/SmaIdigested pHCMC05 This study

Li et al.

Li et al. BMC Genomics 2013 14:130   doi:10.1186/1471-2164-14-130

Open Data