Additional lanes of sequencing data moderately increase saturation and greatly increase 5X CpG coverage. Variation in CpG enrichment (A), saturation (B), and 5X coverage (C) was assessed for 15 lanes of data in the ovarian study corresponding to 7 samples by generating plots of individual lanes and combined replicate lanes for each sample. (D) Average percent deviation of the individual lanes from the combined lane for each sample was plotted for each parameter. Error bars for (D) represent standard error. Asterisks represent Student t-test p < 0.05.
Trimarchi et al. BMC Genomics 2012 13(Suppl 8):S6 doi:10.1186/1471-2164-13-S8-S6