Table 3 |
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Pathogenicity and fitness factors identified from analysis of the genome of M. morganii KT |
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Category |
Niche genes of M. morganii KT |
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Drug resistance |
Ampicillin resistance, ampC-ampR. Metallo-β-lactamases (MBL) MM2254, MM2308, and MM2606. Tellurite resistance operon MM933-1004. Tetracycline resistant, chloramphenicol, acetyltransferase, bicyclomycin, acetyltransferase, kasugamycin tetAJ, catA2, bcr, catB3-like, ksgA. |
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Multidrug resistance and efflux genes (34 genes, Additional File 3: Supplementary table 2). |
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Fimbrial adhesins |
3 MR/P operons, 13 mrpJ paralogous, 1 fimbrial chaperone, 2 UCA (NAF) operons, 1 PMF operon, and 2 other operons (Tables 4, 5). |
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6 putative type IV pili genes hofCB, ppdABCD, and 2 putative trimeric autotransporter secretion genes MM2011, MM2042. |
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Motility/flagellum-related |
Most flagellum-related genes and chemotaxis genes; MM1735-1785, MM1796-1797, and MM1786-1793 (at a single locus) (Additional File 4: Supplementary table 3). |
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Methyl-accepting chemotaxis gene MM0264, aerotaxis gene MM1607, and 9 regulators of flagellar genes umoABCD, rssBA, rcsBCD. |
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T3SS |
Type III secretion system needle complex MM0224-MM0243 (20 genes), and effectors, ipaCBD-chaperone (MM0244-MM0247, 4 genes) (Additional File 5: Supplementary table 4) |
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TCS |
19 potential two-component regulator systems (TCS) were identified. |
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Iron acquisition system |
Heme uptake, ferric, ferrous iron transport, ferric siderophore receptor, B12 transporter, and siderophore iron uptake hmuSTUV, afuABC, feoAB, ireA, btuCD, btuB, and yfeDCBA |
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18 other related genes (fecR, ABC transporters, TonB-dep. receptors) |
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IgA protease |
Zinc metalloprotease, capable of cleaving host Ig, zapABCD (MM1054-MM1056, MM1058) |
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LPS, ECA & capsule |
(1) Release lipid A, induce initial endotoxic shock and enterobacterial common antigen (41 genes, Additional File 6: Supplementary table 5) (2) Protect from phagocytosis, rcsB,C,D,F |
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Haemolysins |
hmpBA (MM2452, MM2453) |
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Ureases |
Urea as a nitrogen source, rapid urea hydrolysis, a cause of stone formation. ureABCEFGD-urea transporter (MM1961-MM1968) |
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Insecticidal& apoptotic toxins |
9 insecticidal toxin: (1) tccB, tccA, and tcdB2 (MM0965-MM0967), (2) xptA1A1C1 (MM1780-1782), (3) tcaC, tccB3, and tcaC (MM1901, MM2567 and MM2570) (Additional File 7: Supplementary table 6) |
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RtxA, XaxAB, intimin/invasion, HlyD toxin secretion, toxin transporter |
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Other genes required for persistence of infection and fitness for infection |
6 superoxide stress genes katA, soxS, sodC,B,A, oxyR (Additional File 8: Supplementary table 7) The phage-shock-protein, pspABC operon (MM0627-MM0629) |
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Ethanolamine utilisation |
Ethanolamine utilisation system, provide carbon and/or nitrogen uptake; eutSPQTDMNEJGHABCLK-pduST-eutR (MM1148-MM1130). (Table 6) |
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Cobalamin biosynthesis |
Cobalamin (vitamin B12) biosynthesis, cbi-cob operon MM1167-MM1187. |
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Chen et al. BMC Genomics 2012 13(Suppl 7):S4 doi:10.1186/1471-2164-13-S7-S4 |
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