Table 2

Mutant Library Generated for ALLA_ECOLI and HCHA_ECOLI proteins of E.coli. The table shows a list of possible double mutants for wild type proteins and corresponding GRAVY values

S.No.

Swissprot ID's

Suggested Patches for Mutation (similar to GGIVLTG)

Patches after mutation

GRAVY value of mutated patch*

(Compare with that of GroES mobile loop)**


1

ALLA_ECOLI

GDVIETQ

GIVIITQ

1.871

ALLA_ECOLI

GDVIETQ

GIVILTQ

1.771

ALLA_ECOLI

GDVIETQ

GIVIVTQ

1.828

ALLA_ECOLI

GDVIETQ

GLVIITQ

1.771

ALLA_ECOLI

GDVIETQ

GLVILTQ

1.671

ALLA_ECOLI

GDVIETQ

GLVIVTQ

1.728

ALLA_ECOLI

GDVIETQ

GVVIITQ

1.828

ALLA_ECOLI

GDVIETQ

GVVILTQ

1.728

ALLA_ECOLI

GDVIETQ

GVVIVTQ

1.785

2

HCHA_ECOLI

GKLFSTG

GILFITG

2.014

HCHA_ECOLI

GKLFSTG

GILFLTG

1.914

HCHA_ECOLI

GKLFSTG

GILFVTG

1.971

HCHA_ECOLI

GKLFSTG

GLLFITG

1.914

HCHA_ECOLI

GKLFSTG

GLLFLTG

1.814

HCHA_ECOLI

GKLFSTG

GLLFVTG

1.871

HCHA_ECOLI

GKLFSTG

GVLFITG

1.971

HCHA_ECOLI

GKLFSTG

GVLFLTG

1.871

HCHA_ECOLI

GKLFSTG

GVLFVTG

1.928


As the single mutations for the patch doesn't make much difference in GRAVY value, so double mutations were considered.

*The GRAVY values are calculated by using ProtParam tool (ExPasy)

**The GRAVY value for mobile loop is = 1.514

Kumar et al. BMC Genomics 2012 13(Suppl 7):S22   doi:10.1186/1471-2164-13-S7-S22

Open Data