Open Access Research article

The construction of a high-density linkage map for identifying SNP markers that are tightly linked to a nuclear-recessive major gene for male sterility in Cryptomeria japonica D. Don

Yoshinari Moriguchi1, Tokuko Ujino-Ihara1, Kentaro Uchiyama1, Norihiro Futamura2, Maki Saito3, Saneyoshi Ueno1, Asako Matsumoto1, Naoki Tani4, Hideaki Taira5, Kenji Shinohara2 and Yoshihiko Tsumura1*

Author Affiliations

1 Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan

2 Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan

3 Toyama Prefectural Agricultural Forestry and Fishieries Research Center, Forestry Research Institute, Yoshimine 3, Tateyama-cho, Nakashinkawagun, Toyama 930-1362, Japan

4 Forestry Division, Japan International Research Center for Agricultural Sciences, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan

5 Graduate School of Science and Technology, Niigata University, Igarashi 2-nocho, Niigata 950-2101, Japan

For all author emails, please log on.

BMC Genomics 2012, 13:95  doi:10.1186/1471-2164-13-95

Published: 16 March 2012

Additional files

Additional file 1:

Candidate markers developed from genes with significant similarity to genes related to male gametophyte development and male sterility in other plant species.

Format: XLS Size: 41KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Candidate markers developed from genes differently expressed between male-fertile and male-sterile individuals.

Format: XLS Size: 30KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Primer information of hrmSNP markers. The method used for marker development has previously been reported by Ujino-Ihara et al. (2010).

Format: XLSX Size: 24KB Download file

Open Data

Additional file 4:

Linkage association between microsatellite markers and a male-sterile gene (ms1) in C. japonica.

Format: XLS Size: 19KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 5:

Primer information for BioMark 48.48 Dynamic Array (Fluidigm) using KASPar assays.

Format: XLSX Size: 18KB Download file

Open Data

Additional file 6:

Results of marker distribution analysis in each marker interval.

Format: XLS Size: 17KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data