PCR analysis of a 648-kb inverted region between genomes of MAP bovine type strain (K-10), and four ovine strains (S397, JTC1074, 1294 and 7565). (A) A diagram showing the inverted region (gray-to-black gradient box) and location of primers used in the PCR analysis. All primers were designed according to the published MAP K-10 genome sequence . (B) PCR results on an ethidium bromide stained agarose gel. Lanes loaded with PCR products amplified with original primer pairs F1 + R1 (lane 8-13) or F2 + R2 (lane 14-19) show no PCR products from the cattle strain (lane 9 and 22) but a 2.1-kb and 3.6-kb band from the sheep strains (lane 10-13 and 23-26), respectively. Lanes with products amplified with switched primer pairs F1 + F2 (lane 14-19) or R1 + R2 (lane 27-32) show a 3.6-kb and 2.3-kb fragment from the cattle strain (lane 15 and 28) but no product from the sheep strains (lane 16-19 and 29-32), respectively. The opposite PCR amplification pattern between the cattle and sheep strains confirmed that this segment is inverted between these 2 genomes.
Bannantine et al. BMC Genomics 2012 13:89 doi:10.1186/1471-2164-13-89