Open Access Research article

C57Bl/6 N mice on a western diet display reduced intestinal and hepatic cholesterol levels despite a plasma hypercholesterolemia

Charles Desmarchelier1*, Christoph Dahlhoff12, Sylvia Keller3, Manuela Sailer1, Gerhard Jahreis3 and Hannelore Daniel1

Author Affiliations

1 Molecular Nutrition Unit, Technische Universität München, Molecular Nutrition Unit, Gregor-Mendel-Strasse 2, 85350 Freising Weihenstephan, Germany

2 PhD Graduate School 'Epigenetics, Imprinting and Nutrition', ZIEL -Research Center for Nutrition and Food Sciences, Technische Universität München (TUM), 85350 Freising Weihenstephan, Germany

3 Friedrich Schiller University, Institute of Nutrition, 07743 Jena, Germany

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BMC Genomics 2012, 13:84  doi:10.1186/1471-2164-13-84

Published: 6 March 2012

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Additional file 1:

Table S1. Primer sequences.

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Additional file 2:

Table S2. Effect of a chronic Western diet on final body weight, cumulative food, energy, water, macronutrient and cholesterol intake and energy assimilation.

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Additional file 3:

Figure S1. Body weight development in mice fed the different diets. Symbols: black diamonds, control diet; grey squares, Western diet. Data are presented as mean ± SEM (n = 12). ** p < 0.01; *** p < 0.001.

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Additional file 4:

Figure S2. Glucose tolerance in mice fed the different diets for 9 weeks. A: A glucose tolerance test was carried out after 9 weeks of dietary intervention following 14 h of food deprivation. Blood was collected from the tail vein 0, 15, 30, 60 and 120 min after an intraperitoneal 20% glucose solution injection (10 ml/kg of body weight) and blood glucose was measured. Symbols: black diamonds, control diet; grey squares, Western diet. B: Area Under the Curve (AUC) calculated from the glucose tolerance test. Black bar: control diet; grey bar: Western diet. Data are presented as mean ± SEM (n = 12). * p < 0.05.

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Additional file 5:

Table S3. Effect of a chronic Western diet on selected blood parameters.

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Table S4. Effect of a chronic Western diet on gene expression in the upper small intestine. Data are presented by ascending q-values. Abbreviations: FC: fold change.

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Table S5. Effect of a chronic Western diet on gene expression in the lower small intestine. Data are presented by ascending q-values. Abbreviations: FC: fold change.

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Additional file 8:

Table S6. Effect of a chronic Western diet on GO Biological Process subsets in the upper small intestine. Data are presented by ascending corrected p-value.

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Additional file 9:

Table S7. Effect of a chronic Western diet on the level of selected phospholipids (PL). Hepatic and intestinal PC levels were detected using LC-MS/MS. Data are presented are mean in μmol/g of protein ± SEM. Abbreviations: C0: DL-carnitine; C2: acetyl-L-carnitine; C3: propionyl-L-carnitine; C4: butyryl-L-carnitine; C4.OH..C3.DC.: hydroxybutyryl-L-carnitine (malonyl-L-carnitine); C5.DC..C6.OH.: glutaryl-L-carnitine (hydroxyhexanoyl-L-carnitine); C5.OH..C3.DC.M.: hydroxyvaleryl-/-isovaleryl-/- methylbutyryl-L-carnitine (methylmalonyl-L-carnitine); C6:1: hexenoyl-L-carnitine; C7-DC: pimelyl-L-carnitine; C16-OH: hydroxyhexadecanoyl-L-carnitine; C16:2: hexadecadienyl-Lcarnitine; C18:1: octadecenoyl-L-carnitine; C18:1-OH: hydroxyoctadecenoyl-L-carnitine; C18:2: octadecadienyl-L-carnitine; FC: fold change; H1: hexose; lysoPC.a.: lysophosphatidylcholine acyl; n.d.: not determined; PC.aa.: phosphatidylcholine diacyl; PC.ae.: phosphatidylcholine acyl-alkyl; SM: sphingomyelin; SM..OH..: hydroxysphingomyeline.

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