Comparison of smoking dependent genes observed by microarray and RNA-Seq. A. Microarray-determined smoking-responsive genes. The data includes all significant genes (Benjamini Hochberg corrected p value < 0.05; Additional file 1, Table S6) with > 1.5-fold different in mean expression level between n = 12 smokers and n = 12 nonsmokers, as determined by microarray. For each probeset the corresponding genes was assessed by RNA-Seq for n = 5 nonsmokers an n = 6 smokers and fold-change by microarray is plotted against the fold-change by RNA-Seq. B. RNA-Seq-determined smoking-responsive genes. The data includes the fold-change of all genes significantly impacted by smoking (uncorrected p < 0.005, 1.5-fold-change cut off), as assessed by RNA-Seq for n = 5 nonsmokers an n = 6 smokers. n = 12 nonsmokers and n = 12 healthy smokers were assessed by microarray and the fold-change for RNA-Seq is plotted against the fold-change for the probeset with largest change.
Hackett et al. BMC Genomics 2012 13:82 doi:10.1186/1471-2164-13-82