Examples of RNA-Seq quantification of small airway epithelium (SAE) expression levels of genes within gene families of ≥ 90% homology. To identify gene families expressed by the SAE, the % identity between gene pairs expressed by the healthy nonsmoker SAE was determined using BLAST, where each gene was blasted against a database of all human RefSeq mRNA . Gene families were defined as genes for which the alignments yielded ≥ 90% identity and the alignment length was at least 50% of both sequences. A. CYP2A6, CYP2A7 and CYP2A13; B. GSTA1, GSTA2, GSTA3 and GSTA5; and C. MT1E, MT1L and MT1M.
Hackett et al. BMC Genomics 2012 13:82 doi:10.1186/1471-2164-13-82