Sexual and asexual oogenesis require the expression of unique and shared sets of genes in the insect Acyrthosiphon pisum
- Equal contributors
1 INRA, UMR 1349 IGEPP, Institut de Génétique Environnement et Protection des Plantes, 35653 Le Rheu cedex, France
2 Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Higashiyama, Myodaiji, Okazaki, 444-8787, Japan
BMC Genomics 2012, 13:76 doi:10.1186/1471-2164-13-76Published: 15 February 2012
Additional file 1:
Transcription of nudel, drp1, clasp1, cbp1 and ACYPI005121 in parthenogenetic ovaries. nudel, drp1, clasp1, cbp1 and ACYPI005121 transcripts localizations were investigated by in situ hybridization on parthenogenetic ovaries including germaria (hollow arrowheads) and in oocytes (black arrowheads). Specific antisense riboprobes gave no signal for these transcripts. Sense riboprobes were used as negative controls. Bar scale: 50 μm.
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Additional file 2:
Transcription of six4, suv4-20, kelch and ACYPI38914 in parthenogenetic ovaries. six4, suv4-20, kelch and ACYPI38914 transcripts localizations were investigated by in situ hybridization on parthenogenetic ovaries including germaria (hollow arrowheads) and in oocytes (black arrowheads). Specific antisense riboprobes showed ubiquitous distribution of these transcripts. Sense riboprobes were used as negative controls. Bar scale: 50 μm.
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Additional file 3:
Quantitative expression of lsd1, lodestar, cyclin J and ACYPI39770 in sexual and asexual embryos measured by microarrays. Transcription levels of lsd-1, lodestar, cyclin-J and ACYPI39770 were measured by microarrays in sexual (full line) and asexual (dot line) embryos. Log 2 of expression value was provided for each developmental stage (18, 19 and 20). The grey area contained the values comprised between the first and third quartile calculated for the 24011 transcripts included in the microarray. Standard errors were measured for the 5 biological replicates.
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Additional file 4:
Experimental design to synchronize the development of pea aphid sexual and asexual embryos. Sexuparae were synchronized at the fourth instar moult in a six hours window. Synchronous sexuparae were randomly separated into two batches; one treated with acetone (A) and one kinoprene (K) 24 h after fourth instar moult. Within each batch, sexuparae were collected 24 h, 48 h and 72 h after treatment, dissected and the 5 most developed embryos were collected for further RNA extraction.
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Additional file 5:
Primer sequences for cDNA amplification and riboprobe synthesis. Specific PCR primers were designed for each of the regulated transcripts in order to amplify cDNA.
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