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Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)

Ernesto Ormeño-Orrillo1*, Pâmela Menna2, Luiz Gonzaga P Almeida3, Francisco Javier Ollero4, Marisa Fabiana Nicolás3, Elisete Pains Rodrigues5, Andre Shigueyoshi Nakatani2, Jesiane Stefânia Silva Batista2, Ligia Maria Oliveira Chueire2, Rangel Celso Souza3, Ana Tereza Ribeiro Vasconcelos3, Manuel Megías4, Mariangela Hungria2 and Esperanza Martínez-Romero1

Author Affiliations

1 Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico

2 Embrapa Soja, C. P. 231, Londrina, Paraná, 86001-970, Brazil

3 Laboratório Nacional de Computação Científica (LNCC), Avenida Getúlio Vargas 333, Petrópolis, Rio de Janeiro, Brazil

4 Universidad de Sevilla, Apdo Postal 874, Sevilla, 41080, Spain

5 Universidade Estadual de Londrina, Cx. Postal 60001, Londrina, Paraná, 86051-990, Brazil

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BMC Genomics 2012, 13:735  doi:10.1186/1471-2164-13-735

Published: 27 December 2012

Additional files

Additional file 1:

Diagram showing the number of orthologous gene clusters shared by R. tropici CIAT 899, Rhizobium sp. PRF 81, R. rhizogenes K84 and R. etli CFN 42. Based on SABIA- and RAST-predicted genes.

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Additional file 2:

Main characteristics of the three strains in this study.

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Additional file 3:

The WaaL O-antigen ligase of Rhizobium tropici CIAT 899. A, Kite-Dolittle hydrophobicity plots of Vibrio cholerae V194 and R. tropici CIAT 899 WaaL proteins. B, R. tropici CIAT 899 WaaL protein represented as a light gray arrow with predicted membrane-spanning regions indicated with dark grey boxes and the position of the Pfam PF04932 conserved domain indicated with a white box. C, Multiple sequence alignment showing conserved regions shared by WaaL proteins. D, Lipolysaccharide profiles of Rhizobium tropici CIAT 899 (wt), its waaL mutant (E4), and the complemented mutant (E4 + waaL).

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Additional file 4:

Induction of indoleacetic acid (IAA) production by flavonoids in R. leucaenae CFN 299 and its 200-kb pSym deletion mutant CFN 299–10.

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