Exon-intron boundaries prediction of Acacia contigs using Medicago truncatula genome as reference. The Blastn alignments of Acacia contigs to M. truncatula v2.0 genome using Phytozome v8 showed in Gbrowser consist of four main types: (A) Acacia contig that shared highly conserved gene structure with M. truncatula genes; (B) Acacia contig that aligned to exonic or untranslated region of M. truncatula genes; (C) Acacia contig that spanned two M. truncatula genes located next to each other in the genome. These two M. truncatula genes are annotated as a single gene in the M. truncatula v3.5 genome and thus matching the Acacia contig alignment; (D) Acacia contig that shared less conserved gene structure with M. truncatula genes.
Wong et al. BMC Genomics 2012 13:726 doi:10.1186/1471-2164-13-726