Phylogenetic relationships between species in section Tomentosae and N. sylvestris as determined by the distribution of repetitive DNA markers. 35S rDNA – yellow; tandem repeats: GRS –pink, NTRS - orange, GRD3 - red, GRD5 - red with yellow centre, HRS60 - blue and white (found only in N. sylvestris). Fluorescence in situ hybridisation of 35S rDNA on Nicotiana chromosomes (yellow signals) with chromosomes counterstained blue with DAPI. Note, dispersed yellow signals, particularly on the fourth pair of N. kawakamii chromosomes. Data and interpretative diagrams are taken from .
Matyášek et al. BMC Genomics 2012 13:722 doi:10.1186/1471-2164-13-722