Table 3

Sequencing coverage

Reference

Read Alignment

Control

T1

T2

T3


None

Total number of reads (million)

15.5

13.7

28.5

29.9


O.s. 6.11

Number of mapped reads (million)

13.4

11.8

24.6

26.4


O.s. 6.1

Complexity (Mb)

131.8

131.5

142.8

142.4


O.s. 6.1

Complexity, homozygous changes2 (Mb)

78.2

77.7

82.7

82.6


De novo3

Number of mapped reads (million)

13.3

11.7

24.5

26.3


De novo

Complexity (Mb)

102.4

102.1

106.9

106.7


De novo

Complexity, homozygous changes2 (Mb)

82.6

82.1

87.4

87.3


1Reference complexity: 380 Mb. 2Complexity for homozygous scoring is calculated according to the following requirements: i) covered at least once in each of the four libraries, ii) covered at least twice in the putative mutant, iii) covered less than 200 times cumulatively in all libraries. 3Reference complexity: 117 Mb.

Monson-Miller et al. BMC Genomics 2012 13:72   doi:10.1186/1471-2164-13-72

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