Distribution of the RESCAN reads used for mutation discovery. Different views of the distribution of the RESCAN reads derived from the control individual "C". A. The red and blue datapoints report respectively the number of total restriction fragment ends available in the genome for the indicated size range (before fractionation) and the number covered by one or more RESCAN. The bar joining the two points highlights the difference. B. Exemplary data for chromosome 5 of rice. The top histogram displays the density distribution of the forward RESCAN reads. The bottom graph plots the count for each RESCAN read vs. the position on chr. 5. The schematic drawing below the chart illustrates the position of the centromere on the chromosome. C. The graph plots read counts for each of the forward RESCAN positions vs the predicted size of the restriction fragment involved. The rescan library for individual T1 has similar properties. Those for individuals T2 and T3 have about double the total number of reads.
Monson-Miller et al. BMC Genomics 2012 13:72 doi:10.1186/1471-2164-13-72