Analyses of two mouse potassium channels transcripts, Kcnb1 and Kcnq3, with putative distal PASs. Panel A. Genomic context of a PTE downstream of the KCNQ3 gene. The interval shown goes from base 133,131,500 to base 133,145,500 of chromosome 8 of the human reference sequence (GRCh37/hg19). The PTE position is indicated with a black arrowhead. The black arrow indicates KCNQ3 direction of transcription. The position of the annotated PAS is indicated with an open arrowhead. On the bottom, the genomic region covered by total RNA deep sequencing reads (RNA seq) from brain (B), testes (T) or heart (H). Panel B. As in Panel A, but for a PTE downstream of the KCNB1 gene. The interval shown goes from base 47,978,000 to base 47,993,000 of chromosome 20. Panel C. Northern blot analysis of total RNA. Three different tissues were tested. B, brain; T, testes; H, heart. On the bottom of each panel, the 28S ribosomal RNA is shown as a loading control. The molecular weight is indicated. For Kcnb1 mRNA, the expected size of the transcript that uses the annotated PAS is of ~3.7 Kb and the expected size of the transcript that uses the predicted distal PAS is of ~11.2 Kb. For Kcnq3 mRNA, the expected size of the transcript that uses the annotated PAS is of ~2.9 Kb and the expected size of the transcript that uses the predicted distal PAS is of ~11.3 Kb.
Morgan et al. BMC Genomics 2012 13:708 doi:10.1186/1471-2164-13-708