Figure 6.

IL-22 gene locus on chromosome 12 in more detail. Generated ChIP-on-Chip data within the tiling array’s coverage range of the IL22 promoter alongside with RefSeq annotation. (A) ChIP peaks represent -10 x log₁₀ of p-values derived from the local comparison of probe signal intensities from tiling microarrays of FOXP3-specific and isotype control IPs from two independent experiments using resting and PMA/ionomycin–stimulated J-FOXP3 cells. Positional overlap of ChIP regions above the p-value threshold (95th percentile) in the two replicate ChIP experiments is summarized in red below. (B) Sequence of summarized ChIP region chr12.119 identified in A and aligned with FOXP3 consensus sequence [8]. Chromosomal coordinates refer to hg18 genomic reference assembly. (C) Fold enrichment of ChIP region chr12.119 in FOXP3-precipitated J-FOXP3 material as determined by genomic quantitative real-time PCR.