Table 3

Differential expression of genes encoding secretory pathway related proteins
DSM code DSM annotation Fold-change B36/N402 P FDR
Protein folding
An02g14800* protein disulfide isomerase A pdiA A. niger 1.72 5.98E-08 3.43E-06
An18g02020* disulfide isomerase tigA A. niger 1.89 1.61E-08 1.17E-06
An01g04600* PDI related protein A prpAA.niger 2.08 2.21E-09 2.40E-07
An16g07620* strong similarity to endoplasmic reticulum oxidising protein Ero1 – S. cerevisiae 1.97 1.02E-08 8.15E-07
An18g04260* similarity to secreted protein HNTME13 from patent WO9839446-A2 – H. sapiens 2.46 1.06E-09 1.28E-07
An18g06470* strong similarity to DnaJ-like protein MTJ1 - Mus musculus 1.48 1.34E-06 3.70E-05
An05g00880* strong similarity to dnaJ protein homolog Scj1 – S. cerevisiae 1.79 1.02E-07 5.04E-06
An01g08420* strong similarity to calcium-binding protein precursor clx1p – S. pombe 2.42 8.96E-10 1.13E-07
An04g02020* strong similarity to cyclophilin cypB – A. nidulans 1.69 1.19E-07 5.60E-06
An01g06670* strong similarity to peptidyl-prolyl isomerase FKBP-21 – N. crassa 1.66 4.71E-07 1.57E-05
An11g04180* dnaK-type molecular chaperone bipA – A. niger 2.32 8.63E-10 1.11E-07
An01g13220* strong similarity to 150 kDa oxygen regulated protein ORP150 - Rattus norvegicus 2.26 1.34E-09 1.58E-07
Signal recognition/cleavage
An04g06890 similarity to 72-kD protein of the signal recognition particle SRP72 - Canis lupus 1.33 1.14E-04 1.37E-03
An15g06470* similarity to signal sequence receptor alpha chain - Canis lupus 1.86 4.49E-08 2.72E-06
An01g00560* strong similarity to signal peptidase subunit Sec11 – S. cerevisiae 1.84 5.70E-07 1.84E-05
An16g07390* strong similarity to endoplasmatic reticulum signal peptidase subunit Spc2 – S. cerevisiae 1.90 7.99E-09 6.74E-07
An09g05420* similarity to signal peptidase subunit Spc3 – S. cerevisiae 1.99 6.90E-09 6.03E-07
Translocation into ER
An03g04340* strong similarity to ER membrane translocation facilitator Sec61 - Yarrowia lipolytica 1.68 9.76E-08 4.90E-06
An01g03820 strong similarity to ER protein-translocation complex subunit Sbh2 – S. cerevisiae 1.62 2.60E-06 6.33E-05
An01g11630* strong similarity to translocation complex component Sss1 – S. cerevisiae 1.71 1.88E-07 8.11E-06
An02g01510* strong similarity to component of the endoplasmic reticulum protein translocation machinery Sec62 – S. cerevisiae 1.57 7.50E-06 1.49E-04
An01g13070* strong similarity to signal recognition particle receptor Sec63 – S. cerevisiae 2.02 2.17E-08 1.47E-06
An16g08830* strong similarity to component of ER protein-translocation subcomplex Sec71 from patent WO9949028-A1 – S. cerevisiae 1.80 3.47E-08 2.17E-06
An15g01670 strong similarity to signal sequence receptor alpha subunit SRP101 - Yarrowia lipolytica 1.29 1.14E-04 1.37E-03
An05g00140* similarity to signal recognition particle receptor beta chain Srp102 – S. cerevisiae 1.39 2.57E-05 4.16E-04
Glycosylation
An02g07650 strong similarity to phosphoglucomutase pgmB – A. nidulans 0.80 5.29E-04 4.81E-03
An03g05940* strong similarity to glutamine-fructose-6-phosphate transaminase Gfa1 – S. cerevisiae 0.66 2.20E-06 5.58E-05
An04g04990* strong similarity to mannose-1-phosphate guanyltransferase MPG1 - Trichoderma reesei 1.46 1.44E-05 2.57E-04
An11g02380* strong similarity to GTP:alpha-D-mannose-1-phosphate guanylyltransferase MPG1 - Hypocrea jecorina 1.35 5.47E-05 7.67E-04
An02g08660 strong similarity to hypothetical protein H04M03.4 - Caenorhabditis elegans 1.25 2.90E-04 2.91E-03
An03g06940* strong similarity to UPD-GlcNAc transporter MNN2-2 - Kluyveromyces lactis 1.35 2.72E-05 4.38E-04
An02g14560* oligosaccharyltransferase alpha subunit OstA – A. niger 2.05 2.27E-09 2.40E-07
An07g04190* strong similarity to dolichyl-diphospho-oligosaccharide--protein glycosyltransferase 48kD chain DDOST - Gallus gallus 1.69 1.03E-07 5.04E-06
An18g03920* strong similarity to defender against apoptotic cell death DAD1 - Homo sapiens 2.04 2.49E-09 2.56E-07
An02g14930* strong similarity to dolichyl-diphosphooligosaccharide-protein glycotransferase gamma chain Ost3 - S. cerevisiae 1.47 1.51E-06 4.08E-05
An16g08570* strong similarity to translation initiation factor 3 47 kDa subunit stt3p – S. pombe 1.78 2.74E-07 1.07E-05
An16g04330* strong similarity to mannose phospho-dolichol synthase dpm1 - Hypocrea jecorina 1.63 2.72E-07 1.07E-05
An01g05200* strong similarity to DPM2 - Mus musculus 1.43 4.84E-05 6.98E-04
An03g04410* strong similarity to UDP-glucose:dolichyl-phosphate glucosyltransferase Alg5 – S. cerevisiae 1.78 3.47E-07 1.26E-05
An02g03240* strong similarity to UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Alg7 – S. cerevisiae 1.92 1.76E-08 1.24E-06
An06g01100* strong similarity to mannosyltransferase Alg1 – S. cerevisiae 1.27 1.50E-04 1.70E-03
An14g05910* strong similarity to mannosyltransferase Alg2 – S. cerevisiae 1.93 2.28E-08 1.53E-06
An18g05910* strong similarity to hypothetical glycosyl transferase SPCC330.08 – S. pombe 1.49 7.14E-05 9.49E-04
An02g14940* strong similarity to human transmembrane protein HTMPN-23 from patent WO9961471-A2 - Homo sapiens 1.49 2.62E-06 6.38E-05
An04g03130 strong similarity to mannosylation protein Lec35 - Cricetulus griseus [putative sequencing error] 1.56 4.25E-07 1.46E-05
An18g02360* strong similarity to Dol-P-Man dependent alpha(1–3) mannosyltransferase Alg3 – S. cerevisiae 1.92 1.74E-08 1.24E-06
An08g07020* similarity to mannosyl transferase Alg9 – S. cerevisiae 1.48 7.80E-06 1.53E-04
An01g08460* strong similarity to the mannosyltransferase Alg12 – S. cerevisiae 1.37 4.69E-04 4.36E-03
An02g12630* strong similarity to glucosyltransferase Alg6 - S. cerevisiae 1.37 1.35E-05 2.44E-04
An04g08820* strong similarity to glucosyltransferase Alg8 – S. cerevisiae 1.24 3.24E-04 3.22E-03
An02g02980* strong similarity to protein influencing Itr1 expression Die2 – S.s cerevisiae 1.42 4.53E-05 6.58E-04
An15g01420* strong similarity to glucosidase I Cwh41 – S. cerevisiae 1.81 2.90E-08 1.84E-06
An18g05620 strong similarity to glucosidase II alpha subunit AAF66685.1 - Homo sapiens 0.80 4.70E-04 4.37E-03
An01g10930* strong similarity to enzyme with sugar transferase activity from patent JP11009276-A - Acremonium sp. 0.45 2.27E-09 2.40E-07
An04g06920* extracellular alpha-glucosidase aglU - Aspergillus niger 0.60 7.55E-08 4.07E-06
An09g03300 strong similarity to alpha-xylosidase XylS - Sulfolobus solfataricus 0.80 4.19E-04 3.98E-03
An09g05880* strong similarity to alpha-glucosidase ModA - Dictyostelium discoideum 1.75 4.77E-08 2.86E-06
An13g00620* strong similarity to 80K protein H precursor G19P1 - Homo sapiens 1.53 1.24E-06 3.47E-05
An07g06430* strong similarity to glycoprotein glucosyltransferase gpt1p – S. pombe 1.64 3.96E-07 1.39E-05
An01g12550 strong similarity to mannosyl-oligosaccharide 1,2-alpha-mannosidase msdS – A. saitoi 0.32 2.29E-11 8.76E-09
An06g01510 strong similarity to class I alpha-mannosidase AAB62720.1 - Spodoptera frugiperda 0.74 4.29E-05 6.31E-04
An12g00340* similarity to alpha 1,2-mannosidase IB - Homo sapiens 1.54 7.61E-06 1.50E-04
An05g01750 strong similarity to alpha-1,6-mannosyltransferase Hoc1 – S. cerevisiae 0.52 1.04E-08 8.30E-07
An11g07490 similarity to alpha-1,6-mannosyltransferase Hoc1 – S. cerevisiae 0.66 8.04E-07 2.46E-05
An15g03330 strong similarity to galactosyltransferase Bed1 – S. cerevisiae 1.42 9.62E-05 1.20E-03
An11g09890* strong similarity to mannosyltransferase 1 PMT1 - Candida albicans 1.36 1.27E-04 1.49E-03
An07g10350* protein O-mannosyl transferase pmtA – A. niger 1.50 4.45E-06 9.69E-05
An16g08490* strong similarity to dolichyl-phosphate-D-mannose--protein O-mannosyltransferase Pmt4 – S. cerevisiae 1.49 1.89E-06 4.90E-05
An15g04810 similarity to alpha-1,3-mannosyltransferase Mnt2 - S. cerevisiae 0.75 2.96E-05 4.66E-04
An02g11720 strong similarity to alpha-mannosidase msd2 – A. nidulans 0.71 1.64E-05 2.85E-04
An01g06500 strong similarity to filamentous growth protein Dfg5 – S. cerevisiae 0.58 1.44E-05 2.56E-04
An02g02660 strong similarity to hypothetical protein Dcw1 – S. cerevisiae 0.78 2.74E-04 2.79E-03
An11g01240* similarity to filamentous growth protein Dfg5 – S. cerevisiae 2.17 1.48E-08 1.10E-06
Protein misfolding (UPR and ERAD associated degradation)
An08g00830 strong similarity to protein phosphatase type 2C Ptc2 – S. cerevisiae 1.31 5.07E-04 4.65E-03
An11g11250* strong similarity to interferon-induced double-stranded RNA-activated protein kinase inhibitor P58 - Homo sapiens 1.80 2.58E-07 1.03E-05
An01g14100* weak similarity to stress protein Herp - Mus musculus 1.61 1.12E-06 3.21E-05
An03g04340* strong similarity to ER membrane translocation facilitator Sec61 – Yarrowia lipolytica 1.68 9.76E-08 4.9E-06
An04g00360* strong similarity to transport vesicle formation protein Sec13 – S. cerevisiae 1.84 1.28E-08 9.9E-07
An15g00640* strong similarity to hypothetical protein GABA-A receptor epsilon subunit – Caenorhabditis elegans 2.03 6.20E-08 3.53E-06
An16g07970* similarity to autocrine motility factor receptor Amfr – Mus musculus 1.60 1.09E-05 2.02E-04
An12g00340* similarity to alpha 1,2-mannosidase IB - Homo sapiens 1.54 7.61E-06 1.50E-04
An15g01420* strong similarity to glucosidase I Cwh41 – S. cerevisiae 1.81 2.90E-08 1.84E-06
An18g06220* strong similarity to alpha-mannosidase Mns1 – S. cerevisiae 2.10 3.47E-06 8.05E-05
An01g12720* similarity to tumour suppressor protein TSA305 from patent WO9928457-A1 - Homo sapiens 1.69 3.91E-07 1.38E-05
Protein complex involved in protein transport
An01g03190 similarity to protein Sec3 – S. cerevisiae 1.30 7.93E-05 1.03E-03
An08g05570* similarity to secretory protein Sec5 – S. cerevisiae 1.54 1.97E-06 5.07E-05
An04g06180 strong similarity to exocyst subunit Sec6 – S.s cerevisiae 1.38 7.87E-05 1.03E-03
An08g07370 similarity to exocyst protein Exo84 – S. cerevisiae 1.42 9.83E-05 1.22E-03
An02g14400* strong similarity to hypothetical protein SPCC338.13 – S. pombe 1.43 8.59E-06 1.66E-04
An16g01630 strong similarity to enoyl reductase of the lovastatin biosynthesis lovC – A.terreus 0.35 1.66E-10 3.53E-08
An04g08690* similarity to polynucleotide sequence SEQ ID NO:3913 from patent WO200058473-A2 - Homo sapiens 1.37 7.88E-05 1.03E-03
An02g07090* strong similarity to ASNA1 product arsenite translocating ATPase - Homo sapiens 1.30 1.46E-04 1.66E-03
An01g14250* strong similarity to delta subunit of the coatomer delta-coat protein CopD - Bos taurus 1.46 3.66E-06 8.35E-05
An08g01250* weak similarity to COP1-interacting protein 7 CIP7 - Arabidopsis thaliana 1.75 4.69E-07 1.57E-05
An16g05370* similarity to zinc-finger protein Glo3 - S. cerevisiae 1.59 1.28E-05 2.34E-04
An16g02460* strong similarity to alpha subunit of the coatomer complex Ret1 – S. cerevisiae 1.67 2.40E-07 9.68E-06
An01g14260* strong similarity to delta subunit of the coatomer delta-coat protein CopD - Bos taurus [deleted ORF] 1.51 1.15E-06 3.27E-05
An12g04830* strong similarity to coatomer protein zeta chain Ret3 – S. cerevisiae 1.47 7.15E-06 1.44E-04
An07g06030* strong similarity to coatomer gamma subunit 2 copg2 - Homo sapiens 1.65 4.32E-06 9.45E-05
An02g05870* strong similarity to coatomer beta subunit copB2 - Homo sapiens [putative frameshift] 1.50 1.17E-04 1.40E-03
An01g04040* secretion-associated GTP-binding protein sarA – A. niger 1.27 1.99E-04 2.13E-03
An08g03270* strong similarity to beta-COP Sec26 – S. cerevisiae 1.48 2.84E-06 6.82E-05
An04g00360* strong similarity to transport vesicle formation protein Sec13 – S. cerevisiae 1.84 1.28E-08 9.90E-07
An02g01690* strong similarity to p150 component of the COPII coat of secretory pathway vesicles Sec31 – S. cerevisiae 1.60 2.05E-07 8.64E-06
An01g04730* strong similarity to secretory protein Sec23 – S. cerevisiae 1.62 2.83E-07 1.08E-05
An08g10650* strong similarity to transport protein Sec24 – S. cerevisiae 1.55 6.97E-07 2.18E-05
An16g03320* strong similarity to transport protein Sec24A - Homo sapiens 1.56 2.27E-06 5.69E-05
An15g01520* strong similarity to multidomain vesicle coat protein Sec16 – S. cerevisiae 1.53 1.74E-06 4.55E-05
ER to Golgi and intra-Golgi transport
An08g03590* strong similarity to precursor of protein Emp24 – S. cerevisiae 1.39 7.73E-06 1.52E-04
An09g05490* strong similarity to COP-coated vesicle membrane protein P24 homolog lbrA - Polysphondylium pallidum 1.42 4.30E-06 9.43E-05
An07g09160 strong similarity to pattern formation protein cni - Drosophila melanogaster 1.28 1.30E-04 1.52E-03
An01g08870* strong similarity to component of COPII-coated vesicles Erv25 - S. cerevisiae 1.39 7.23E-06 1.45E-04
An08g03960* strong similarity to hypothetical endoplasmic reticulum associated protein – S. pombe 1.55 7.36E-06 1.47E-04
An03g04940* strong similarity to Erv41 - S. cerevisiae 2.12 1.20E-08 9.44E-07
An01g04320* strong similarity to COPII vesicle coat component protein Erv46 - S. cerevisiae 2.12 7.61E-09 6.57E-07
An02g02830 strong similarity to protein RER1 - Homo sapiens 1.30 5.22E-05 7.42E-04
An07g02190 strong similarity to protein Sec7 - S. cerevisiae 1.44 9.50E-06 1.81E-04
An08g06780* strong similarity to transport protein Uso1 - S. cerevisiae 1.52 2.93E-06 7.00E-05
An18g06440 strong similarity to COPII vesicle component Yip3 - S. cerevisiae 1.67 2.58E-06 6.30E-05
An04g01780* strong similarity to hypothetical protein YAR002c-a - S. cerevisiae 1.54 1.03E-06 3.00E-05
An04g08830* similarity to Golgi membrane protein Emp47 - S. cerevisiae 1.69 9.30E-07 2.78E-05
An02g04250* similarity to protein p58 - Rattus norvegicus 1.77 2.01E-08 1.39E-06
An04g01990* similarity to protein ZW10 homolog HZW10 - Homo sapiens 1.32 1.06E-04 1.29E-03
An04g06090 similarity to geranylgeranyltransferase type-II alpha chain Bet4 - S. cerevisiae 0.77 2.69E-04 2.75E-03
An08g00290* strong similarity to golgin-160 related protein Rud3 - S. cerevisiae 1.52 1.11E-05 2.05E-04
An08g06330* strong similarity to epsilon-COP - Cricetulus griseus 1.39 1.84E-05 3.14E-04
An07g07340* strong similarity to luminal ER-protein retention receptor ERD2 - Kluyveromyces marxianus 1.65 2.00E-07 8.46E-06
Other processes in the secretory pathway
An07g02170* similarity to transport protein Bos1 - S. cerevisiae 1.91 1.65E-08 1.18E-06
An15g01380* strong similarity to Synaptobrevin homolog v-SNARE Sec22 - S. cerevisiae 1.30 2.74E-04 2.78E-03
An18g02490* strong similarity to ARF guanine-nucleotide exchange factor 2 Gea2 - S. cerevisiae 1.31 1.52E-04 1.71E-03
An07g08220* strong similarity to clathrin associated epsin 2A - Homo sapiens 1.39 1.58E-05 2.76E-04
An02g08450* secretory gene nsfA - Aspergillus niger 1.27 2.86E-04 2.89E-03
An02g14450* secretory pathway Ca2+−ATPase pmrA - Aspergillus niger 1.51 3.12E-06 7.38E-05
An16g08470* similarity to hypothetical cell growth regulator OS-9 - Homo sapiens 1.76 8.57E-07 2.58E-05
An02g03460* similarity to hypothetical protein YIL041w - S. cerevisiae 1.31 1.49E-04 1.68E-03
An04g02070 strong similarity to clathrin heavy chain - Bos taurus 1.30 1.09E-04 1.33E-03
An06g01200* strong similarity to endosomal protein Emp70 - S. cerevisiae 1.55 1.37E-06 3.77E-05
An01g11960 similarity to brefeldin A resistance protein Bfr1 - S. cerevisiae 1.44 1.27E-05 2.33E-04
An04g01950* strong similarity to zinc-metalloprotease Ste24 - S. cerevisiae 1.63 1.61E-07 7.12E-06

DSM code: ORF identifier in A. niger CBS 513.88 genome sequence [3]. Genes in bold are also found in maltose/xylose transcriptomic comparison [23]. * Indicates genes that were also identified in strains with constitutively active hacACA[36].

Kwon et al.

Kwon et al. BMC Genomics 2012 13:701   doi:10.1186/1471-2164-13-701

Open Data