Table 5

Primers for Eimeria tenella protease genes for the amplified coding sequences
Protease gene Primer Primer sequence 5 to 3
Aspartic Proteases
Eimepsin 1 F ATC ACC ACA CCA CCA TGG G
R CAA GAT TCG AGC AGT TCT CAG CA
Eimepsin 2 F AGC AAA CAG CTG CAG ATG TTC C
R CGA AAA TAG GTC TAG GGG CCC
Eimepsin 3 F AGA AGT CCT TTC CTC CGT CAC G
R GGC GAA GTG TAT TGA AAG CTC G
Cysteine Proteases
Cathepsin B F TGA CGT GGG AAG CAG AAG TGT C
R ACT GTA TGC ACT CGT GGC GAA A
Cathepsin L F CAA CCA ACA AGG TCA CTC TTA C
R CCC TGG AGG GCC CCC GTG CTC G
Cathepsin C1 F AAC GGA AGT GGA GAA GAC AGC A
R CGG GGT CAA TGA AGG AAG TTT G
Cathepsin C2 F GGC TTT GAA GTT TGG CAG CG
R TGC ATC TGA GCA GCA GAA GAG
Cathepsin C3 F CGC TCA GGA GTA CAA CTA CGT GGG TGG
R GCT GCT GCA CAA GCA GCG CTG CTC TGC C
Calpain F CGA CTG CTC CTT CCT CTC
R GCA TCC TTT AGC TGC TGG
Ubiquitinyl hydrolase 1 F CTG GCT CTT GAA TGT GCT GCA T
R CTT CCA TTT CGT GCC ATT CG
Ubiquitinyl hydrolase 2 F TTG GAC TTC AGC CCG AGG AG
R CCG CTC GTC AAA TCC CAA AG
Ubiquitinyl hydrolase 3 F AGG TGG TGC CCC TCG ACA TA
R TTC TGC TGC CGT GCT CTT TG
Ubiquitinyl hydrolase 4 F GTG AAT GTG GGC AGC ACC AG
R GCT TTC CTG CAA GGC GAA GA
Ubiquitinyl hydrolase 5 F TCT CTT CCA GGG GCA GTA CAG G
R GCT GCT CGT AGG CCA TAT TTG AAC
Ubiquitinyl hydrolase 6 F GGT GGC CAG CAT AGA CGA GAG T
R CCG TAG GTT TGC TGC GAT CTC T
Ubiquitinyl hydrolase 7 F CGA CAG TCC CGT TGT TGC TG
R AGC AAG CCG GGG AAA GAG AC
Ubiquitinyl hydrolase 8 F TCC TGG AGG CCT TGA GCA GT
R TGC TGC TCA CTG GGA TGT GG
OTU protease F ATG GAC GTA GCA ATC CGT TAC
R GTT GCT GCA CAT AGT CCC AAG
Pyroglutamyl peptidase F ATG GGA CAA CCT ACA GGC GAG
R GAG GAA GTT ACA AAC GAA GCA GC
Metallo Proteases
Aminopeptidase N 1 F CAA GCA GTA CAC TCC AGC AAC TC
R GAA GCG TCA GGT GTT CGT ATT CC
Aminopeptidase N 2 F GAT ACA TCG CAA GGA CTA CAG C
R CAT TGT GCG GAA GGA GTC TG
ATP-dependant Zn protease 1 F CTT CGA CCA GCT GAA GAT CCT G
R TGT CTC CGC GCT AAT GCT G
ATP-dependant Zn protease 2 F CTC AAT GGT GGA TTT CAG CAA G
R CAT CAT TCC CTG GCT TGC G
ATP-dependant Zn protease 3 F GTC AAG AAA GCA GAT TGT CAG G
R CTC GAC AGC CAT CTC AAA GTC
CAAX prenyl protease F GAA TGT CCC AGG AGA GCT ATG C
R GTT CAC AGC GCA CAG AAT TGG
Insulysin 1 F CAC TTC CTG GAG CAC ATG G
R GTG CAG CCT GTC GAA GAT G
Insulysin 2 F CCG CGG TAG CCT CTCA GC
R CTC CGC TGT TGC TCA CAT CTT C
Insulysin 3 F GAT TGA CGC CGA GCA CCA G
R GCT AGG CGA GCT GCT TCA TCC
Insulysin 4 F GTC GTG CAG AGA GGC TGG
R GAG GTA GCT GAG TCG GAG GG
Insulysin 5 F ATG ATA TTT CTG AAG TCT GC
R GAG GAT TCA AAA GAT GGT C
Leucine aminopeptidase F CAA GAT CTA GTT GTT TGA GGA GCC C
R GCT GTT CCA CTT CAC TCG TCT TC
O-sialoglycoprotease F GCA AAT GTG ACG CAG CTG ATT CG
R CGA ACT TGA GGG CCA TTT GCT C
S2P-like protease F GCC CTC GTA TAA CAG CAA C
R GAA CGA CTA ACT TCC TCT TGC
Serine Proteases
Trypsin 1 F GTA GGC AAC GGA ACT CCA GC
R CGA GGA CTA CAA CTG TCT CC
Trypsin 2 F ATG GAA GCG TCT GGT TGG GAC
R GCT TTT GCT GCA TGC ACT C
Trypsin 3 F GAC AAA CAA GTT CAA CGA GCA CTG
R GCT TCC CTC TCC GCA GAA TTG
Subtilisin 1 F TAA TTA CCT CCA TCC CGA ACT G
R CCA GAA TCT TCA GCG CCA TCA C
Subtilisin 2 F GCA GCA GCA AAT GTT GAA GAC CC
R ATA AGT GCT GCT GCC AAC CAC C
Subtilisin 3 F AGA GCT TTT GTC CGT GGT GGA G
R AAA GAC CCC GAA AAC CAA TGC T
Subtilisin 4 F CCT TTG TGG CGT GTT CGT GAG
R CCA GCA GAA GCA GTA CCG TGG CC
Subtilisin 5 F TTG AAG CCG ACA GGA CGT GG
R CCG CGT AGT CAA GAG CAG GGA T
Subtilisin 6 F AGC GGC TGC GAC TTG AAC C
R CCG TAG CCG CCG TAG GAG TT
Prolyl endopeptidase F ACA GCC AGG CAC ATC AAT GGT
R GCC AAA CCC AAG CCC AGA TAG
Clp protease F GCT GCA CTT CCA GAA GCG G
R CCT CCG CAG AGA AGA CTT TGC
Rhomboid protease 1 F GGT TGT CCG CAC GTT GGC AG
R CGA AGA TAA CAG GCA CGC AAG ATG

Katrib et al.

Katrib et al. BMC Genomics 2012 13:685   doi:10.1186/1471-2164-13-685

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