Table 2

Expression of protease genes in merozoites, gametocytes, unsporulated oocysts and sporulated oocysts of Eimeria tenella
Protease group cDNA product Stage specific expression Predicted amplicon size Actual amplicon size Genbank accession number
Aspartic Proteases M G UO SO (bp) (bp)
Eimepsin 1 Oocyst Specific 357 659 AJ293829
Eimepsin 2 Gametocyte Specific 467 467 JX503496
Eimepsin 3 Merozoite Upregulated 578 578 JX503497
Cysteine proteases
Cathepsin B Downregulated in Sporulated Oocyst 656 656 JN641867.1
Cathepsin L Downregulated in Gametocytes 330 300 JX503498
Cathepsin C1 Merozoite Upregulated 547 547 JX503499
Cathepsin C2 Gametocyte Upregulated 273 273 JX503500
Cathepsin C3 Gametocyte and Unsporulated Oocyst Upregulated 436 436 JX546580
Calpain Merozoite Upregulated 831 806 JX503501
Ubiquitinyl hydrolase 1 Merozoite Upregulated 548 548 JX503503
Ubiquitinyl hydrolase 2 Gametocyte Upregulated 395 395 JX503504
Ubiquitinyl hydrolase 3 Merozoite, possibly Gametocyte Upregulated 698 698 JX503505
Ubiquitinyl hydrolase 4 Merozoite Upregulated 499 499 JX503506
Ubiquitinyl hydrolase 5 Gametocyte Upregulated 674 719 JX503507
Ubiquitinyl hydrolase 6 - 546 - -
Ubiquitinyl hydrolase 7 - 529 - -
Ubiquitinyl hydrolase 8 - 303 - -
OTU protease - 802 - -
Pyroglutamyl peptidase Gametocyte Upregulated 735 771 JX503502
Metalloproteases
Aminopeptidase N 1 Downregulated in Merozoite 602 602 JX503508
Aminopeptidase N 2 Gametocyte Upregulated 636 636 JX503509
ATP-dependant Zn protease 1 Downregulated in Sporulated Oocyst 604 604 XM_001238661.1
ATP-dependant Zn protease 2 Merozoite Upregulated 389 389 JX503516
ATP-dependant Zn protease 3 Downregulated in Sporulated Oocyst 567 1045 JX503517
CAAX prenyl protease Merozoite Upregulated 368 368 JX503518
Insulysin 1 Merozoite Upregulated 294 294 JX503510
Insulysin 2 Merozoite Upregulated 416 416 JX503511
Insulysin 3 Oocyst Specific 402 697 JX503512
Insulysin 4 Gametocyte Upregulated 367 367 JX503513
Insulysin 5 Merozoite Upregulated 202 202 *
Leucyl aminopeptidase Merozoite, possibly Gametocyte Upregulated 804 859 JX503514
O-sialoglycoprotease Merozoite Upregulated 412 412 JX503515
S2P-like protease Gametocyte Specific 616 1027 JX503519
Serine proteases
Trypsin 1 Gametocyte Specific 613 613 JX503520
Trypsin 2 Gametocyte Upregulated 721 721 JX503521
Trypsin 3 Merozoite Upregulated 654 849 JN987371.1
Subtilisin 1 Gametocyte Specific 260 260 XM_001238722.1
Subtilisin 2 Gametocyte Specific 690 690 XM_001238654.1
Subtilisin 3 Merozoite Upregulated 1171 1084 JX503522
Subtilisin 4 Gametocyte and Unsporulated Oocyst Upregulated 566 566 JX503523
Subtilisin 5 Gametocyte Specific 360 414 JX503524
Subtilisin 6 - 365 - -
Prolyl endopeptidase Downregulated in Sporulated Oocyst 534 534 JX503526
Clp protease Merozoite Upregulated 413 241 JX503525
Rhomboid protease 1 Merozoite Upregulated 554 554 JN987619.1

M Merozoites, G Gametocytes, UO Unsporulated Oocysts, SO Sporulated Oocysts.

* = short sequence not accepted for submission.

Katrib et al.

Katrib et al. BMC Genomics 2012 13:685   doi:10.1186/1471-2164-13-685

Open Data