PCR results for validation of 50 pea and 50 S.sclerotiorum pyrosequence-classified EST contigs against a mix of host and pathogen interaction cDNA, host cDNA only and pathogen cDNA only. All 50 PCR pea primer sets amplified the expected amplicons from the pea-S. sclerotiorum and pea cDNA samples. Forty-seven of the 50 PCR S. sclerotiorum primer sets amplified expected amplicons from the pea-S. sclerotiorum and S. sclerotiorum cDNA samples, one primer pair failed to amplify from either cDNA and the remaining two only amplified product from the pea-S. sclerotiorum cDNA possibly indicating interaction induced transcripts.
Zhuang et al. BMC Genomics 2012 13:668 doi:10.1186/1471-2164-13-668