Open Access Highly Accessed Research article

Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library

Xian Pan1, Bingkun Lei1, Nan Zhou1, Biwei Feng1, Wei Yao1, Xin Zhao2, Yao Yu1* and Hong Lu1*

Author Affiliations

1 State Key Laboratory of Genetic Engineering, School of Life Sciences, and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China

2 Department of Animal Science, McGill University, Ste. Anne de Bellevue, Quebec, H9X 3V9, Canada

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BMC Genomics 2012, 13:662  doi:10.1186/1471-2164-13-662

Published: 23 November 2012

Additional files

Additional file 1:

Table S1. List of genes whose deletions exhibited sensitivity to DNA damage reagents during the second round of screen. Table S2. GO profiling of 52 genes whose deletion mutants showed strong sensitivity to DNA damage reagents (P ≤ 0.05). Table S3. Flow cytometry analysis of 37 mutants. Table S4. Primers used for real time PCR analysis in this study. Figure S1. Spot assay of 52 deletions. Exponentially growing cells, WT or deletions, were harvested and 5-fold serial dilutions were spotted on the plates supplemented with DNA damage reagents. The plates were photographed after 3~4 days of incubation at 32°C. Figure S2. Flow cytometry analysis of deletions in “2C” group. Figure S3. Flow cytometry analysis of deletions in “1C” group. Figure S4. Flow cytometry analysis of deletions in “W4C” group. Figure S5. Flow cytometry analysis of deletions in “S4C” group.

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