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Open Access Research article

Nuclear genomic control of naturally occurring variation in mitochondrial function in Drosophila melanogaster

Patricia Jumbo-Lucioni14, Su Bu1, Susan T Harbison2, Juanita C Slaughter1, Trudy FC Mackay3, Douglas R Moellering1* and Maria De Luca1*

Author affiliations

1 Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL, 35294, USA

2 Laboratory of Systems Genetics, National Heart Lung and Blood Institute, Bethesda, MD, 20892-1654, USA

3 Department of Genetics, North Carolina State University, Raleigh, NC, 27695, USA

4 Present address: Department of Biological Sciences, Vanderbilt University, Nashville, TN, 37232, USA

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Citation and License

BMC Genomics 2012, 13:659  doi:10.1186/1471-2164-13-659

Published: 22 November 2012

Abstract

Background

Mitochondria are organelles found in nearly all eukaryotic cells that play a crucial role in cellular survival and function. Mitochondrial function is under the control of nuclear and mitochondrial genomes. While the latter has been the focus of most genetic research, we remain largely ignorant about the nuclear-encoded genomic control of inter-individual variability in mitochondrial function. Here, we used Drosophila melanogaster as our model organism to address this question.

Results

We quantified mitochondrial state 3 and state 4 respiration rates and P:O ratio in mitochondria isolated from the thoraces of 40 sequenced inbred lines of the Drosophila Genetic Reference Panel. We found significant within-population genetic variability for all mitochondrial traits. Hence, we performed genome-wide association mapping and identified 141 single nucleotide polymorphisms (SNPs) associated with differences in mitochondrial respiration and efficiency (P ≤1 × 10-5). Gene-centered regression models showed that 2–3 SNPs can explain 31, 13, and 18% of the phenotypic variation in state 3, state 4, and P:O ratio, respectively. Most of the genes tagged by the SNPs are involved in organ development, second messenger-mediated signaling pathways, and cytoskeleton remodeling. One of these genes, sallimus (sls), encodes a component of the muscle sarcomere. We confirmed the direct effect of sls on mitochondrial respiration using two viable mutants and their coisogenic wild-type strain. Furthermore, correlation network analysis revealed that sls functions as a transcriptional hub in a co-regulated module associated with mitochondrial respiration and is connected to CG7834, which is predicted to encode a protein with mitochondrial electron transfer flavoprotein activity. This latter finding was also verified in the sls mutants.

Conclusions

Our results provide novel insights into the genetic factors regulating natural variation in mitochondrial function in D. melanogaster. The integrative genomic approach used in our study allowed us to identify sls as a novel hub gene responsible for the regulation of mitochondrial respiration in muscle sarcomere and to provide evidence that sls might act via the electron transfer flavoprotein/ubiquinone oxidoreductase complex.