Sub-cellular localization of NNV capsid protein and BiP protein in NNV-infected GF-1 cells. Grouper fin cell line GF-1 was infected with NNV at MOI of 10. At 24 hr post-infection, the cells were fixed and co-stained with rabbit anti-NNV capsid and mouse anti-BiP, followed by Texas Red conjugated anti-rabbit IgG and Fluorscein conjugated mouse IgG. The Nucleus was visualized by counter-staining with 4', 6-diamidino-2-phenylindole (DAPI). The NNV capsid protein (A) is shown to co-localize with BiP protein (B) in distinct area within the cytoplasm as indicated by the merged image (C). Uninfected GF1 cell was indicated by white arrow.
Lu et al. BMC Genomics 2012 13:651 doi:10.1186/1471-2164-13-651