Figure 2.

Ascophyllum nodosum extracts did not rescue the freezing sensitive phenotype of p5cs1 mutants: (A) Petri Plate Freezing Tolerance Assay with wild-type Arabidopsis (left partition of Petri plate) and p5cs1 mutants (right partition of Petri plate) treated with (i) Control (ii) Ascophyllum nodosum extract (ANE) and (iii) lipophilic component of ANE (LPC), (B) survival rate at different freezing temperatures of wild type Arabidopsis and p5cs1 mutant plants treated with ANE or LPC in Petri Plates, (C) proline accumulation of p5cs1 mutants treated with ANE or LPC in response to freezing in peat pellets, and (D) Phenotypic responses of p5cs1 mutant plants treated with (i) water control (ii) ANE (1.0 g L-1) and (iii) LPC (1.0 g L-1) to a temperature of −2°C for 24 h in Peat pellet freezing assay. Bars are the means ± SE.

Nair et al. BMC Genomics 2012 13:643   doi:10.1186/1471-2164-13-643
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