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Open Access Research article

Transcriptional and metabolomic analysis of Ascophyllum nodosum mediated freezing tolerance in Arabidopsis thaliana

Prasanth Nair1, Saveetha Kandasamy1, Junzeng Zhang2, Xiuhong Ji2, Chris Kirby3, Bernhard Benkel1, Mark D Hodges4, Alan T Critchley5, David Hiltz5 and Balakrishnan Prithiviraj1*

Author Affiliations

1 Department of Environmental Sciences, Dalhousie Agricultural Campus, Dalhousie University, Truro, NS, B2N 5E3, Canada

2 Institute for Nutrisciences and Health, National Research Council of Canada, Charlottetown, PEI, C1A 4P3, Canada

3 Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 550 University Avenue, Charlottetown, PE, C1A 4N6, Canada

4 Atlantic Food and Horticulture Research Centre, Agriculture and Agri-Food Canada, Kentville, NS, B4N 1J5, Canada

5 Acadian Seaplants Limited, 30 Brown Ave., Dartmouth, NS, B3B 1X8, Canada

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BMC Genomics 2012, 13:643  doi:10.1186/1471-2164-13-643

Published: 21 November 2012

Abstract

Background

We have previously shown that lipophilic components (LPC) of the brown seaweed Ascophyllum nodosum (ANE) improved freezing tolerance in Arabidopsis thaliana. However, the mechanism(s) of this induced freezing stress tolerance is largely unknown. Here, we investigated LPC induced changes in the transcriptome and metabolome of A. thaliana undergoing freezing stress.

Results

Gene expression studies revealed that the accumulation of proline was mediated by an increase in the expression of the proline synthesis genes P5CS1 and P5CS2 and a marginal reduction in the expression of the proline dehydrogenase (ProDH) gene. Moreover, LPC application significantly increased the concentration of total soluble sugars in the cytosol in response to freezing stress. Arabidopsis sfr4 mutant plants, defective in the accumulation of free sugars, treated with LPC, exhibited freezing sensitivity similar to that of untreated controls. The 1H NMR metabolite profile of LPC-treated Arabidopsis plants exposed to freezing stress revealed a spectrum dominated by chemical shifts (δ) representing soluble sugars, sugar alcohols, organic acids and lipophilic components like fatty acids, as compared to control plants. Additionally, 2D NMR spectra suggested an increase in the degree of unsaturation of fatty acids in LPC treated plants under freezing stress. These results were supported by global transcriptome analysis. Transcriptome analysis revealed that LPC treatment altered the expression of 1113 genes (5%) in comparison with untreated plants. A total of 463 genes (2%) were up regulated while 650 genes (3%) were down regulated.

Conclusion

Taken together, the results of the experiments presented in this paper provide evidence to support LPC mediated freezing tolerance enhancement through a combination of the priming of plants for the increased accumulation of osmoprotectants and alteration of cellular fatty acid composition.

Keywords:
Arabidopsis thaliana; Ascophyllum nodosum; Freezing tolerance; Chemical priming; Soluble sugars; Metabolite profiling; Microarray analysis