Western blot analysis with α-Rubisco antibody. (A) Western blot of five micrograms of proteins extracted from the two biological replicates of Bowman (Bow) and Bowman-Rph15 (Bow-Rph15) at 4 days after mock inoculation (C) or after inoculation with leaf rust spores (I) were separated on 12% SDS-PAGE and hybridized with a Rubisco antibody. Bands representing the main Rubisco degradation forms are numbered from 1 to 5 on the Western right side. (B) Quantification data of Rubisco degradation forms; evaluation of degradation forms was conducted for the five bands indicated in (A) in Bowman (Bow) and Bowman-Rph15 (Bow-Rph15) under control (C) or after inoculation with leaf rust spores (I) conditions. The data are the mean of three independent experiments. Different letters on the bars indicate significant difference P-values (P < 0.05) of the t-TESTs performed comparing control and inoculated samples.
Bernardo et al. BMC Genomics 2012 13:642 doi:10.1186/1471-2164-13-642