Samples description and experimental design for next-generation sequencing.A) 13 different tissues and conditions used for RNA purification. RAC : White young roots; FTN : Young leaves and stems; FTB : Leaves infected with Botrytis cinerea LR18; FTS: Leaves from water stressed plants; NDB: Dormant axillary buds (vegetative meristem); DBO: Active axillary buds (vegetative meristem); IFL: Floral bud at floral meristem transition; IMO: Floral meristem and early floral organs (sepal, petal, stamens and carpels) development; BFL: closed flower; DET: Stamens at microsporogenesis and microgametogenesis stages; OFT: open flower; SEN: senescent flower; CYN: rose hip from pollination up to early pigmentation. B, Sequencing and assembly strategy. Illumina reads were assembled using edena and combined with the trimmed 454 reads using TGICL to generate the final clusters assembly.
Dubois et al. BMC Genomics 2012 13:638 doi:10.1186/1471-2164-13-638