Bioinformatics identification of putative LEF1/TCF target genes. The diagram presents the successive steps to select putative LEF1/TCF targets, beginning from the group of human-rat orthologs in the Ensembl database. Groups of genes are in rectangles, and computational procedures are in diamonds. In the blue frame, the crossing of our in silico-selected genes with known Wnt/β-catenin targets is shown. The p values (Fisher’s Exact test) confirmed the enrichment of genes with at least one and at least two LEF1/TCF binding sites (predicted with Matinspector using Genomatix family V$LEFF) with the known targets.
Wisniewska et al. BMC Genomics 2012 13:635 doi:10.1186/1471-2164-13-635