Overview of the construction of the C. pneumoniae ORFeome. The PCR products of the amplified ORFs were used in the first step of a BP reaction to create an Entry library, followed by PCR and sequencing for technical confirmation. For expression of recombinant proteins, the Entry library was used for LR reactions. Additionally, an Y2H library was generated for validation by functional analysis.
Maier et al. BMC Genomics 2012 13:632 doi:10.1186/1471-2164-13-632