Table 1

Strains, plasmids, and cell lines used in this study

Strain, cell line, or plasmid

Relevant characteristicsa

Source or reference


Escherichia coli

DH5α

F-, ɸ 80 lacZ DM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (rK- mK+) supE44 thi-1 ΔgyrA96 relA1

Laboratory stock

SY327

araD Δ(lac pro) argE(Am) recA56 rifr nalA, λ pir

[75]

Burkholderia cenocepacia

J2315

Epidemic strain ET12 clone, CF clinical isolate

P.A. Sokol

K56-2

Epidemic strain ET12 clone, CF clinical isolate

BCRRCb

MH1K

K56-2 ΔBCAL1674-6

[30]

JST19

MH1K ΔBCAL0124

This study

JST71

MH1K ΔBCAS0186

This study

JST75

MH1K ΔBCAM2837

This study

JST128

MH1K ΔBCAL1726

This study

JST130

MH1K ΔBCAM0276

This study

JST132

MH1K ΔBCAM0411

This study

JST134

MH1K ΔBCAM2446

This study

JST136

MH1K ΔBCAM0434-5

This study

JST190

MH1K ΔBCAL0340

This study

JST194

MH1K ΔBCAM1679

This study

Macrophage cell lines

ANA-1

C57BL/6 murine bone marrow-derived macrophages

[76]

RAW 264.7

BALB/c murine macrophage cell line

ATCCc

Plasmid

pDA12

oripBBR1, TetR, mob+, Pdhfr

[28]

pDA42

pDA12, eGFP

[28]

pDAI-SceI-SacBN

oripBBR1, TetR, mob+, Pdhfr, sacB, encodes I-SceI endonuclease

[30]

pDelbcsM

pGPI-SceI with regions flanking BCAL0340

[77]

pGPI-SceI

oriR6K, TpR, mob+, carries I-SceI cut site

[74]

pJT25

pUC19 with K56-2 rDNA (23S, 16S, 5S), ApR

This study

pJT31

pGPI-SceI with regions flanking BCAL0124

This study

pJT41

pGPI-SceI with regions flanking BCAS0186

This study

pJT42

pGPI-SceI with regions flanking BCAM2837

This study

pJT45

pGPI-SceI with regions flanking BCAL1726

This study

pJT46

pGPI-SceI with regions flanking BCAM0276

This study

pJT47

pGPI-SceI with regions flanking BCAM0411

This study

pJT48

pGPI-SceI with regions flanking BCAM2446

This study

pJT49

pGPI-SceI with regions flanking BCAM0434-5

This study

pJT52

pDA12, BCAL1726

This study

pJT54

pDA12, BCAM0411

This study

pJT57

pDA12, BCAL0124

This study

pJT71

pDA12, BCAL0340

This study

pJT72

pGPI-SceI with regions flanking BCAM1679

This study

pRK201

RK2 derivative, KmR, mob+, tra+, oricolE1

[68]

pUC19

oripMB1, ApR, mob+, Plac

[78]


a Tp, trimethoprim; Tet, tetracycline; Ap, ampicillin; Km, kanamycin

b B. cepacia complex Research and Referral Repository for Canadian CF Clinics

c American Type Culture Collection

Tolman and Valvano BMC Genomics 2012 13:63   doi:10.1186/1471-2164-13-63

Open Data