Comparative bacterial infection, host cytotoxicity, and normalized replication of parental and mutant B. cenocepacia. Bacterial infection and cytotoxicity (A) are shown as a percentage of total cells. Cytotoxicity was normalized against cell death in uninfected cells. 50 000 cells were counted in each of three independent experiments. Standard error bars are indicated. Significance was determined using one-way ANOVA and Dunnett's Multiple Comparison Test. Mutants demonstrating significant difference from the wild-type are indicated (*p < 0.05, **p < 0.01, ***p < 0.001). The ratio of dead cells: infected cells (B) was used to correct intracellular replication determined by the gentamicin protection assay to give the total expected ratio of intracellular bacteria in the absence of cell death (C). Mutants with a ratio in (B) less than zero are unchanged in (C).
Tolman and Valvano BMC Genomics 2012 13:63 doi:10.1186/1471-2164-13-63