Figure 1.

Experimental design showing pertinent steps in the TGE protocol including the indexing PCR during which barcodes are incorporated into separate DNA libraries.A) standard post-capture TGE, 96 captures are required as barcodes are incorporated during second PCR after capture and prior to sequencing, B) pre-capture multiplexing with pools of 12 (pre-capture 12a and pre-capture 12b), 8 captures required as barcodes are incorporated when adaptors are ligated prior to the first PCR and capture, and C) pre-capture multiplexing with pools of 16 (pre-capture 16), 6 captures required.

Shearer et al. BMC Genomics 2012 13:618   doi:10.1186/1471-2164-13-618
Download authors' original image