Table 2

Copy number of ATDs-rolC in the double transgenic lines, and genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.

Transgenic line

Copy number

Genomic insertion locus (scaffold:position)*

Score

E-value

Transcript (POPTR_)


N82-2

1

18:5,952,389

841.7

0

---


N82-3

1

7:3,347,471

854.3

0

0007s05190

no functional annotation


N82-4

1

8:2,414,980

580.2

5.0e-164

0008s04320

Cyclin, N-terminal domain


N82-5

1

607:11,508

132.9

1.4e-29

0607s00230

no functional annotation


18:4,994,998

165.4

1.7e-39

0018s05440

Ankyrin Repeat-Containing

N82-7

4

7:9,643,105

71.6

2.5e-11

---

2:14,852,606

41.0

2.2e-2

---

n.d.**


N82-8

1

18:1,568,406

1463.8

0

POPTR_0018s01710

or

F-Box/Leucine Rich Repeat Protein

3:13,577,759

1551.2

0

POPTR_0003s13180 Heat Shock Protein 90


N82-10

2

2:10,740,018

612.2

5.3e-174

0002s14460

Zinc-finger double stranded

5:9,290,567

91.5

3.4e-17

0005s12520

GRAS family transcription factor


N82-11

1

16:13,606,858

879.5

0

POPTR_0016s14360

Elongation factor P, C-terminal


N82-12

2

17:7,632,882

44.6

9.8e-3

---

n.d.**

---


N82-14

2

3:13,408,914

322.3

1.9e-89

---

n.d.**

---


N82-15

1

2:21,183,733

199.7

6.8e-50

---


N92-1

1

15:6,453,477

286.2

1.6e-75

---


N92-2

1

2:10,740,357

706.4

0

---


N92-3

1

11:16,469,472

39.2

2.0e-3

POPTR_0011s13850 (COBRA-like protein)


N92-4

1

10:15,737,708

515.2

2.1e-144

---


N92-5

1

10:19,481,904

417.9

4.4e-115

---


N92-6

n.d.**

n.d.**


N95-1

1

n.d.**


N95-2

2

25:155,725

1490.9

0

POPTR_0025s00400 (CYTH domain)

15:824,499

48.2

4.2e-4

---


N95-3

1

9:9,143,423

291.6

3.0e-77

POPTR_0009s10720 (Arylacetamide deacetylase)


N95-4

1

17:7,632,882

44.6

3.2e-3

---


N95-5

1

16:7,726,062

562.1

1.4e-158

---


N95-6

n.d.**

n.d.**


In bold: blast-results with high e-values. In BLAST-analyses where more than one hit was given, either the one with lower e-value or when similar, both hits are shown.

*based on BLAST-results against the genome sequence of P. trichocarpa v2.0 (Phytozome v7.0; http://www.phytozome.net/poplar webcite). Successful positioning of blasted sequence on the physical map of P. trichocarpa was assigned to the Populus-aspen genome because of the high collinearity between the P. trichocarpa and P. tremula/P.tremuloides genomes [49].

**n.d. = not determined.

Fladung and Polak BMC Genomics 2012 13:61   doi:10.1186/1471-2164-13-61

Open Data