Open Access Research article

Transcriptomic and phylogenetic analysis of Culex pipiens quinquefasciatus for three detoxification gene families

Liangzhen Yan12, Pengcheng Yang1, Feng Jiang1, Na Cui1, Enbo Ma2, Chuanling Qiao1 and Feng Cui1*

Author Affiliations

1 State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

2 Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, 030006, China

For all author emails, please log on.

BMC Genomics 2012, 13:609  doi:10.1186/1471-2164-13-609

Published: 10 November 2012

Additional files

Additional file 1:

Summary of the carboxylcholinesterase genes ofCulex pipiens quinquefasciatus. TPM, number of transcripts per million clean tags. When the expression of a gene was not detected, TPM was set to 0.01.

Format: XLS Size: 51KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Summary of the cytochrome P450 monooxygenase genes ofCulex pipiens quinquefasciatus. TPM, see Additional file 1.

Format: XLS Size: 69KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Summary of the glutathione S-transferase genes ofCulex pipiens quinquefasciatus. TPM, see Additional file 1.

Format: XLS Size: 26KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Distribution of the total tags in each DGE library.

Format: TIFF Size: 821KB Download file

Open Data

Additional file 5:

Pearson correlation analysis of the DGE libraries. Dots in the figures indicate individual tag entities. TPM (Transcripts Per Million clean tags) indicates the number of transcript copies in every 1 million clean tags. A–F, correlation between the four developmental stages of the SG strain; G, correlation between third instar larvae of the SG and S-lab strains.

Format: TIFF Size: 1.4MB Download file

Open Data

Additional file 6:

Relationship between the number of identified genes and sequencing amount.

Format: TIFF Size: 5.5MB Download file

Open Data

Additional file 7:

Enriched GO function groups*and KEGG pathways involved by genes specifically expressing in various life stages ofCulex pipiens quinquefasciatus.*GO function groups include three main categories: biological process (BP), molecular function (MF), and cellular component (CC). The p value represented the difference between the specifically expressed gene group and the total 13,314 gene group approximated by chi-square test. Fisher exact test is used when any expected count value is below 5. Benjamini is the multiple-testing correction of the p value by FDR.

Format: DOC Size: 48KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 8:

Differentially expressed genes between the parathion-resistant and susceptible larvae ofCulex pipiens quinquefasciatus. TPM, see Additional file 1.

Format: XLS Size: 344KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data