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Open Access Highly Accessed Research article

High-throughput novel microsatellite marker of faba bean via next generation sequencing

Tao Yang1, Shi-ying Bao2, Rebecca Ford3, Teng-jiao Jia1, Jian-ping Guan1, Yu-hua He2, Xue-lian Sun1, Jun-ye Jiang1, Jun-jie Hao4, Xiao-yan Zhang4 and Xu-xiao Zong1*

Author affiliations

1 Institute of Crop Science, The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

2 Institute of Grain Crops, Yunnan Academy of Agricultural Sciences, Kunming, 650205, China

3 Department of Agriculture and Food Systems, Melbourne School of Land and Environment, The University of Melbourne, Melbourne, Victoria, 3010, Australia

4 Qingdao Academy of Agricultural Sciences, Qingdao, 266100, China

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Citation and License

BMC Genomics 2012, 13:602  doi:10.1186/1471-2164-13-602

Published: 8 November 2012

Abstract

Background

Faba bean (Vicia faba L.) is an important food legume crop, grown for human consumption globally including in China, Turkey, Egypt and Ethiopia. Although genetic gain has been made through conventional selection and breeding efforts, this could be substantially improved through the application of molecular methods. For this, a set of reliable molecular markers representative of the entire genome is required.

Results

A library with 125,559 putative SSR sequences was constructed and characterized for repeat type and length from a mixed genome of 247 spring and winter sown faba bean genotypes using 454 sequencing. A suit of 28,503 primer pair sequences were designed and 150 were randomly selected for validation. Of these, 94 produced reproducible amplicons that were polymorphic among 32 faba bean genotypes selected from diverse geographical locations. The number of alleles per locus ranged from 2 to 8, the expected heterozygocities ranged from 0.0000 to 1.0000, and the observed heterozygosities ranged from 0.0908 to 0.8410. The validation by UPGMA cluster analysis of 32 genotypes based on Nei's genetic distance, showed high quality and effectiveness of those novel SSR markers developed via next generation sequencing technology.

Conclusions

Large scale SSR marker development was successfully achieved using next generation sequencing of the V. faba genome. These novel markers are valuable for constructing genetic linkage maps, future QTL mapping, and marker-assisted trait selection in faba bean breeding efforts.

Keywords:
Microsatellite markers; Next generation sequencing; Marker development; Vicia faba L.