Figure 4.

Verification of differential methylation of four arbitrarily chosen genes. a. Methylation differences between Red Junglefowl and White Leghorn in three different tissues, estimated by methylation sensitive high resolution melting (MS-HRM) analysis. The table shows the normalized fluorescent intensities together with the statistical analysis of 6-8 individual samples per population, at a temperature where positive and negative methylation controls showed highest intensity difference (Tmd). The right column shows the number of SNPs present in the PCR-product amplified by the primers for the region analysed. b-d, The average methylation level of the promoter regions of three of the genes, FUCA1 (b), PCDHAC1 (c) and RUFY3 (d), in blood samples from F8-generation intercross birds between Red Junglefowl and White Leghorns. The birds differed as indicated in their genotype on an SNP-marker close to the differentially methylated locus.

N├Ątt et al. BMC Genomics 2012 13:59   doi:10.1186/1471-2164-13-59
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