Open Access Highly Accessed Research article

Combining proteomics and transcriptome sequencing to identify active plant-cell-wall-degrading enzymes in a leaf beetle

Roy Kirsch1, Natalie Wielsch2, Heiko Vogel1, Aleš Svatoš2, David G Heckel1 and Yannick Pauchet1*

Author Affiliations

1 Entomology department, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745, Jena, Germany

2 Mass spectrometry Research Group, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745, Jena, Germany

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BMC Genomics 2012, 13:587  doi:10.1186/1471-2164-13-587

Published: 1 November 2012

Additional files

Additional file 1:

Figure S1. Chromatogram of the anion exchange chromatography of P. cochleariae gut content proteins; Figure S2, CMC and pectin zymograms; Figure S3 , GH11s amino acid alignment; Figure S4, GH28s amino acid alignment; Figure S5, GH45s amino acid alignment; Table S1, possible N-glycosylation sites found for each putative P. cochleariae PCWDE.

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Additional file 2:

Details of the protein identifications obtained from the proteomics approach after mass spectrometry analyses. For each analysis performed (LC-MS/MS or LC-MSE, searching either against a viridiplantae of the P. cochleariae database), data are presented in a separated spreadsheet.

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