Combining proteomics and transcriptome sequencing to identify active plant-cell-wall-degrading enzymes in a leaf beetle
- Equal contributors
1 Entomology department, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745, Jena, Germany
2 Mass spectrometry Research Group, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745, Jena, Germany
BMC Genomics 2012, 13:587 doi:10.1186/1471-2164-13-587Published: 1 November 2012
Additional file 1:
Figure S1. Chromatogram of the anion exchange chromatography of P. cochleariae gut content proteins; Figure S2, CMC and pectin zymograms; Figure S3 , GH11s amino acid alignment; Figure S4, GH28s amino acid alignment; Figure S5, GH45s amino acid alignment; Table S1, possible N-glycosylation sites found for each putative P. cochleariae PCWDE.
Format: DOC Size: 6.6MB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 2:
Details of the protein identifications obtained from the proteomics approach after mass spectrometry analyses. For each analysis performed (LC-MS/MS or LC-MSE, searching either against a viridiplantae of the P. cochleariae database), data are presented in a separated spreadsheet.
Format: XLS Size: 207KB Download file
This file can be viewed with: Microsoft Excel Viewer